PcMtr, an aromatic and neutral aliphatic amino acid permease of Penicillium chrysogenum.

Cloning and characterization of an aromatic amino acid and leucine permease of Penicillium chrysogenum.

The gene encoding the amino acid permease ArlP (Aromatic and leucine Permease) was isolated from the filamentous fungus Penicillium chrysogenum after PCR using degenerated oligonucleotides based on conserved regions of fungal amino acid permeases. The cDNA clone was used for expression of the permease in Saccharomyces cerevisiae M4054, which is defective in the general amino acid permease Gap1....

Uptake of the -Lactam Precursor -Aminoadipic Acid in Penicillium chrysogenum Is Mediated by the Acidic and the General Amino Acid Permease

External addition of the -lactam precursor -aminoadipic acid to the filamentous fungus Penicillium chrysogenum leads to an increased intracellular -aminoadipic acid concentration and an increase in penicillin production. The exact route for -aminoadipic acid uptake is not known, although the general amino acid and acidic amino acid permeases have been implicated in this process. Their correspon...

Basic amino acid transport in plasma membrane vesicles of Penicillium chrysogenum.

The characteristics of the basic amino acid permease (system VI) of the filamentous fungus Penicillium chrysogenum were studied in plasma membranes fused with liposomes containing the beef heart mitochondrial cytochrome c oxidase. In the presence of reduced cytochrome c, the hybrid membranes accumulated the basic amino acids arginine and lysine. Inhibition studies with analogs revealed a narrow...

University of Groningen Uptake of the β-Lactam Precursor α-Aminoadipic Acid in Penicillium chrysogenum Is Mediated by the Acidic and the General Amino Acid Permease

Accumulation of intracellular inorganic sulfate by Penicillium chrysogenum.

The cell membranes of microorganisms appear to be rather complex, metabolically active entities, equipped with many specific active transport mechanisms which mediate the uptake and accumulation of a variety of compounds. Bacteria have been shown to accumulate amino acids (Cohen and Rickenberg, 1956; Gale, 1947; 1953), carbohydrates (Cohen and Monod, 1957; Rickenberg et al., 1956), and inorgani...